simple chip plus sonication chromatin ip kit (Cell Signaling Technology Inc)
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Simple Chip Plus Sonication Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chromatin Immunoprecipitation:Article Title: IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints Article Snippet: .. Samples were processed further using Cell Signaling Article Title: The beneficial androgenic action of steroidal aromatase inactivators in estrogen-dependent breast cancer after failure of nonsteroidal drugs Article Snippet: .. Confluent cultures (80%) were shifted to PRF for 24 h and then treated with various drugs at a concentration of 10 –7 M (as shown in Fig. ) or left untreated in 5% PRF-CT for 2 h. Following treatment, ChIP assay was performed as described by Morelli et al. and using a simple Article Title: COUP-TFII plays a role in cAMP-induced Schwann cell differentiation and in vitro myelination by up-regulating Krox20. Article Snippet: SangHeum Han and Young Hee Kim equally contributed to this study.. Abbreviations: AAV, Adenoassociated virus; AKT, protein kinase B; ANOVA, analysis of variance; BSA, bovine serum albumin; cAMP, cyclic adenosine monophosphate; ChipPCR, chromatin immunoprecipitationpolymerase chain reaction; ChipqPCR, chromatin immunoprecipitationquantitative polymerase chain reaction; CNS, central nervous system; COUPTFII, chicken ovalbumin upstream promotertranscription factor II; DMEM, Dulbecco's Modified Eagle's Medium; DRG, dorsal root ganglion; ECM, extracellular matrix; Egr2, early growth response protein 2; ERK, extracellular signalregulated kinase; FBS, fetal bovine serum; GA, geldanamycin; Gab1, Grb2associated binder 1; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GPR126, G proteincoupled receptor 126; Hsp90, heat shock protein 90; LPA, lysophosphatidic acid; MAG, myelinassociated glycoprotein; MAPK, mitogenactivated protein kinase; MBP, myelin basic protein; MPZ, myelin protein zero; MSE8, myelinating Schwann cell element 8; mTOR, mammalian target of rapamycin; mTORC1, mTOR complex 1; NRG1, neuregulin1; Oct6, octamerbinding transcription factor 6; PAF, paraformaldehyde; PBS, phosphatebuffered saline; PDL, polyDlysine hydrobromide; PDNF, polyvinylidene fluoride; PI3K, phosphoinositide 3kinase; PMSF, phenylmethylsulfonyl fluoride; PNS, peripheral nervous system; Pou3f1, POU domain class 3 transcription factor; PTEN, phosphatase and tensin homolog; RIPA, radioimmunoprecipitation assay; RT, room temperature; RTqPCR, reverse transcriptaserealtime polymerase chain reaction; RXRγ, retinoid X receptor γ; SCs, Schwann cells; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; SEM, standard error of mean; Sox10, SRYrelated HMGbox10; SREBP1c, sterol response element binding protein1c; TBST, Trisbuffered saline0.1% Tween 20; TFs, transcription factors; Tuj1, neuronspecific class III βtubulin; YY1, Yin Yang 1.. 1Department of Biochemistry, DongA University College of Medicine, Busan, South Korea 2Peripheral Neuropathy Research Center, DongA University, Busan, South Korea 3Department of Translational Biomedical Sciences, DongA University Graduate School, Busan, South Korea 4Department of Laboratory Medicine, DongA University College of Medicine, Busan, South Korea 5Department of Molecular Neuroscience, DongA University College of Medicine, Busan, South Korea Article Title: IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints. Article Snippet: How IRF7 promotes autoimmune B cell responses and systemic autoimmunity is unclear.. Analysis of spontaneous SLE-prone mice deficient in IRF7 uncovered the IRF7 role in regulating autoimmune germinal center (GC), plasma cell (PC), and autoantibody responses and disease.. IRF7, however, was dispensable for foreign antigen–driven GC, PC, and antibody responses. Article Title: SMURF2-Mediated Ubiquitination and Degradation of HOXA5 Augments Endoplasmic Reticulum Stress in Alveolar Epithelial Type II Cells Induced by LPS. Article Snippet: Acute lung injury (ALI) is a clinical syndrome characterized by pulmonary inflammatory and endoplasmic reticulum stress (ERS).. Nevertheless, the involvement of Homeobox A5 (HOXA5) in ERS of alveolar epithelial type II (ATII) cells remains unclear.. RT-qPCR and western blot were employed to detect the expression of HOXA5, SMAD-specific E3 ubiquitin protein ligase 2 (SMURF2), Fatty-acid-binding protein 4 (FABP4), inflammatory factors, and ERS-related proteins. Sonication:Article Title: IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints Article Snippet: .. Samples were processed further using Cell Signaling Article Title: The beneficial androgenic action of steroidal aromatase inactivators in estrogen-dependent breast cancer after failure of nonsteroidal drugs Article Snippet: .. Confluent cultures (80%) were shifted to PRF for 24 h and then treated with various drugs at a concentration of 10 –7 M (as shown in Fig. ) or left untreated in 5% PRF-CT for 2 h. Following treatment, ChIP assay was performed as described by Morelli et al. and using a simple Article Title: COUP-TFII plays a role in cAMP-induced Schwann cell differentiation and in vitro myelination by up-regulating Krox20. Article Snippet: SangHeum Han and Young Hee Kim equally contributed to this study.. Abbreviations: AAV, Adenoassociated virus; AKT, protein kinase B; ANOVA, analysis of variance; BSA, bovine serum albumin; cAMP, cyclic adenosine monophosphate; ChipPCR, chromatin immunoprecipitationpolymerase chain reaction; ChipqPCR, chromatin immunoprecipitationquantitative polymerase chain reaction; CNS, central nervous system; COUPTFII, chicken ovalbumin upstream promotertranscription factor II; DMEM, Dulbecco's Modified Eagle's Medium; DRG, dorsal root ganglion; ECM, extracellular matrix; Egr2, early growth response protein 2; ERK, extracellular signalregulated kinase; FBS, fetal bovine serum; GA, geldanamycin; Gab1, Grb2associated binder 1; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GPR126, G proteincoupled receptor 126; Hsp90, heat shock protein 90; LPA, lysophosphatidic acid; MAG, myelinassociated glycoprotein; MAPK, mitogenactivated protein kinase; MBP, myelin basic protein; MPZ, myelin protein zero; MSE8, myelinating Schwann cell element 8; mTOR, mammalian target of rapamycin; mTORC1, mTOR complex 1; NRG1, neuregulin1; Oct6, octamerbinding transcription factor 6; PAF, paraformaldehyde; PBS, phosphatebuffered saline; PDL, polyDlysine hydrobromide; PDNF, polyvinylidene fluoride; PI3K, phosphoinositide 3kinase; PMSF, phenylmethylsulfonyl fluoride; PNS, peripheral nervous system; Pou3f1, POU domain class 3 transcription factor; PTEN, phosphatase and tensin homolog; RIPA, radioimmunoprecipitation assay; RT, room temperature; RTqPCR, reverse transcriptaserealtime polymerase chain reaction; RXRγ, retinoid X receptor γ; SCs, Schwann cells; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; SEM, standard error of mean; Sox10, SRYrelated HMGbox10; SREBP1c, sterol response element binding protein1c; TBST, Trisbuffered saline0.1% Tween 20; TFs, transcription factors; Tuj1, neuronspecific class III βtubulin; YY1, Yin Yang 1.. 1Department of Biochemistry, DongA University College of Medicine, Busan, South Korea 2Peripheral Neuropathy Research Center, DongA University, Busan, South Korea 3Department of Translational Biomedical Sciences, DongA University Graduate School, Busan, South Korea 4Department of Laboratory Medicine, DongA University College of Medicine, Busan, South Korea 5Department of Molecular Neuroscience, DongA University College of Medicine, Busan, South Korea Article Title: IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints. Article Snippet: How IRF7 promotes autoimmune B cell responses and systemic autoimmunity is unclear.. Analysis of spontaneous SLE-prone mice deficient in IRF7 uncovered the IRF7 role in regulating autoimmune germinal center (GC), plasma cell (PC), and autoantibody responses and disease.. IRF7, however, was dispensable for foreign antigen–driven GC, PC, and antibody responses. Article Title: SMURF2-Mediated Ubiquitination and Degradation of HOXA5 Augments Endoplasmic Reticulum Stress in Alveolar Epithelial Type II Cells Induced by LPS. Article Snippet: Acute lung injury (ALI) is a clinical syndrome characterized by pulmonary inflammatory and endoplasmic reticulum stress (ERS).. Nevertheless, the involvement of Homeobox A5 (HOXA5) in ERS of alveolar epithelial type II (ATII) cells remains unclear.. RT-qPCR and western blot were employed to detect the expression of HOXA5, SMAD-specific E3 ubiquitin protein ligase 2 (SMURF2), Fatty-acid-binding protein 4 (FABP4), inflammatory factors, and ERS-related proteins. Concentration Assay:Article Title: The beneficial androgenic action of steroidal aromatase inactivators in estrogen-dependent breast cancer after failure of nonsteroidal drugs Article Snippet: .. Confluent cultures (80%) were shifted to PRF for 24 h and then treated with various drugs at a concentration of 10 –7 M (as shown in Fig. ) or left untreated in 5% PRF-CT for 2 h. Following treatment, ChIP assay was performed as described by Morelli et al. and using a simple Real-time Polymerase Chain Reaction:Article Title: COUP-TFII plays a role in cAMP-induced Schwann cell differentiation and in vitro myelination by up-regulating Krox20. Article Snippet: SangHeum Han and Young Hee Kim equally contributed to this study.. Abbreviations: AAV, Adenoassociated virus; AKT, protein kinase B; ANOVA, analysis of variance; BSA, bovine serum albumin; cAMP, cyclic adenosine monophosphate; ChipPCR, chromatin immunoprecipitationpolymerase chain reaction; ChipqPCR, chromatin immunoprecipitationquantitative polymerase chain reaction; CNS, central nervous system; COUPTFII, chicken ovalbumin upstream promotertranscription factor II; DMEM, Dulbecco's Modified Eagle's Medium; DRG, dorsal root ganglion; ECM, extracellular matrix; Egr2, early growth response protein 2; ERK, extracellular signalregulated kinase; FBS, fetal bovine serum; GA, geldanamycin; Gab1, Grb2associated binder 1; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GPR126, G proteincoupled receptor 126; Hsp90, heat shock protein 90; LPA, lysophosphatidic acid; MAG, myelinassociated glycoprotein; MAPK, mitogenactivated protein kinase; MBP, myelin basic protein; MPZ, myelin protein zero; MSE8, myelinating Schwann cell element 8; mTOR, mammalian target of rapamycin; mTORC1, mTOR complex 1; NRG1, neuregulin1; Oct6, octamerbinding transcription factor 6; PAF, paraformaldehyde; PBS, phosphatebuffered saline; PDL, polyDlysine hydrobromide; PDNF, polyvinylidene fluoride; PI3K, phosphoinositide 3kinase; PMSF, phenylmethylsulfonyl fluoride; PNS, peripheral nervous system; Pou3f1, POU domain class 3 transcription factor; PTEN, phosphatase and tensin homolog; RIPA, radioimmunoprecipitation assay; RT, room temperature; RTqPCR, reverse transcriptaserealtime polymerase chain reaction; RXRγ, retinoid X receptor γ; SCs, Schwann cells; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; SEM, standard error of mean; Sox10, SRYrelated HMGbox10; SREBP1c, sterol response element binding protein1c; TBST, Trisbuffered saline0.1% Tween 20; TFs, transcription factors; Tuj1, neuronspecific class III βtubulin; YY1, Yin Yang 1.. 1Department of Biochemistry, DongA University College of Medicine, Busan, South Korea 2Peripheral Neuropathy Research Center, DongA University, Busan, South Korea 3Department of Translational Biomedical Sciences, DongA University Graduate School, Busan, South Korea 4Department of Laboratory Medicine, DongA University College of Medicine, Busan, South Korea 5Department of Molecular Neuroscience, DongA University College of Medicine, Busan, South Korea Polymerase Chain Reaction:Article Title: COUP-TFII plays a role in cAMP-induced Schwann cell differentiation and in vitro myelination by up-regulating Krox20. Article Snippet: SangHeum Han and Young Hee Kim equally contributed to this study.. Abbreviations: AAV, Adenoassociated virus; AKT, protein kinase B; ANOVA, analysis of variance; BSA, bovine serum albumin; cAMP, cyclic adenosine monophosphate; ChipPCR, chromatin immunoprecipitationpolymerase chain reaction; ChipqPCR, chromatin immunoprecipitationquantitative polymerase chain reaction; CNS, central nervous system; COUPTFII, chicken ovalbumin upstream promotertranscription factor II; DMEM, Dulbecco's Modified Eagle's Medium; DRG, dorsal root ganglion; ECM, extracellular matrix; Egr2, early growth response protein 2; ERK, extracellular signalregulated kinase; FBS, fetal bovine serum; GA, geldanamycin; Gab1, Grb2associated binder 1; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GPR126, G proteincoupled receptor 126; Hsp90, heat shock protein 90; LPA, lysophosphatidic acid; MAG, myelinassociated glycoprotein; MAPK, mitogenactivated protein kinase; MBP, myelin basic protein; MPZ, myelin protein zero; MSE8, myelinating Schwann cell element 8; mTOR, mammalian target of rapamycin; mTORC1, mTOR complex 1; NRG1, neuregulin1; Oct6, octamerbinding transcription factor 6; PAF, paraformaldehyde; PBS, phosphatebuffered saline; PDL, polyDlysine hydrobromide; PDNF, polyvinylidene fluoride; PI3K, phosphoinositide 3kinase; PMSF, phenylmethylsulfonyl fluoride; PNS, peripheral nervous system; Pou3f1, POU domain class 3 transcription factor; PTEN, phosphatase and tensin homolog; RIPA, radioimmunoprecipitation assay; RT, room temperature; RTqPCR, reverse transcriptaserealtime polymerase chain reaction; RXRγ, retinoid X receptor γ; SCs, Schwann cells; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; SEM, standard error of mean; Sox10, SRYrelated HMGbox10; SREBP1c, sterol response element binding protein1c; TBST, Trisbuffered saline0.1% Tween 20; TFs, transcription factors; Tuj1, neuronspecific class III βtubulin; YY1, Yin Yang 1.. 1Department of Biochemistry, DongA University College of Medicine, Busan, South Korea 2Peripheral Neuropathy Research Center, DongA University, Busan, South Korea 3Department of Translational Biomedical Sciences, DongA University Graduate School, Busan, South Korea 4Department of Laboratory Medicine, DongA University College of Medicine, Busan, South Korea 5Department of Molecular Neuroscience, DongA University College of Medicine, Busan, South Korea ChIP-qPCR:Article Title: COUP-TFII plays a role in cAMP-induced Schwann cell differentiation and in vitro myelination by up-regulating Krox20. Article Snippet: SangHeum Han and Young Hee Kim equally contributed to this study.. Abbreviations: AAV, Adenoassociated virus; AKT, protein kinase B; ANOVA, analysis of variance; BSA, bovine serum albumin; cAMP, cyclic adenosine monophosphate; ChipPCR, chromatin immunoprecipitationpolymerase chain reaction; ChipqPCR, chromatin immunoprecipitationquantitative polymerase chain reaction; CNS, central nervous system; COUPTFII, chicken ovalbumin upstream promotertranscription factor II; DMEM, Dulbecco's Modified Eagle's Medium; DRG, dorsal root ganglion; ECM, extracellular matrix; Egr2, early growth response protein 2; ERK, extracellular signalregulated kinase; FBS, fetal bovine serum; GA, geldanamycin; Gab1, Grb2associated binder 1; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GPR126, G proteincoupled receptor 126; Hsp90, heat shock protein 90; LPA, lysophosphatidic acid; MAG, myelinassociated glycoprotein; MAPK, mitogenactivated protein kinase; MBP, myelin basic protein; MPZ, myelin protein zero; MSE8, myelinating Schwann cell element 8; mTOR, mammalian target of rapamycin; mTORC1, mTOR complex 1; NRG1, neuregulin1; Oct6, octamerbinding transcription factor 6; PAF, paraformaldehyde; PBS, phosphatebuffered saline; PDL, polyDlysine hydrobromide; PDNF, polyvinylidene fluoride; PI3K, phosphoinositide 3kinase; PMSF, phenylmethylsulfonyl fluoride; PNS, peripheral nervous system; Pou3f1, POU domain class 3 transcription factor; PTEN, phosphatase and tensin homolog; RIPA, radioimmunoprecipitation assay; RT, room temperature; RTqPCR, reverse transcriptaserealtime polymerase chain reaction; RXRγ, retinoid X receptor γ; SCs, Schwann cells; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; SEM, standard error of mean; Sox10, SRYrelated HMGbox10; SREBP1c, sterol response element binding protein1c; TBST, Trisbuffered saline0.1% Tween 20; TFs, transcription factors; Tuj1, neuronspecific class III βtubulin; YY1, Yin Yang 1.. 1Department of Biochemistry, DongA University College of Medicine, Busan, South Korea 2Peripheral Neuropathy Research Center, DongA University, Busan, South Korea 3Department of Translational Biomedical Sciences, DongA University Graduate School, Busan, South Korea 4Department of Laboratory Medicine, DongA University College of Medicine, Busan, South Korea 5Department of Molecular Neuroscience, DongA University College of Medicine, Busan, South Korea Binding Assay:Article Title: SMURF2-Mediated Ubiquitination and Degradation of HOXA5 Augments Endoplasmic Reticulum Stress in Alveolar Epithelial Type II Cells Induced by LPS. Article Snippet: Acute lung injury (ALI) is a clinical syndrome characterized by pulmonary inflammatory and endoplasmic reticulum stress (ERS).. Nevertheless, the involvement of Homeobox A5 (HOXA5) in ERS of alveolar epithelial type II (ATII) cells remains unclear.. RT-qPCR and western blot were employed to detect the expression of HOXA5, SMAD-specific E3 ubiquitin protein ligase 2 (SMURF2), Fatty-acid-binding protein 4 (FABP4), inflammatory factors, and ERS-related proteins. |
